This cross-sectional study aimed to look into the sensitivity and specificity of the Xpert Ultra assay to diagnose childhood pulmonary TB using stool specimens. Under this study, children under 15 years of age with signs and symptoms suggestive of TB (presumptive TB patients) were enrolled in four hospitals. Stool specimens and induced sputum were collected from presumptive child TB patients. These specimens were then transported to icddr,b’s mycobacteriology laboratory on the same day of specimen collection for laboratory tests. At the laboratory, after the processing of specimens, smear microscopy, the Xpert MTB/RIF Ultra assay (Xpert Ultra), and culture tests were conducted. The data gathered from the laboratory tests was thus analyzed to infer the study findings.
The study found stool to be a superior specimen to induced sputum in childhood TB diagnosis by using Xpert Ultra. Among stool and induced sputum specimens, the positivity of Xpert Ultra was higher in stool specimens (13.4%) than induced sputum specimens (6.3%).
- To determine the sensitivity of the Xpert Ultra test to diagnose childhood pulmonary TB compared to bacteriologically positive TB cases using stool specimens
- To determine the specificity of the Xpert Ultra test to rule out childhood pulmonary TB compared to bacteriologically negative TB cases using stool specimens
(Dhaka Medical College Hospital;
Shaheed Suhrawardy Medical College and Hospital;
Sir Salimullah Medical College and Mitford Hospital; and
icddr,b Dhaka Hospital)
National Tuberculosis Control Program (NTP)
DATE OF RESULTS DISSEMINATION
24 November 2019
Dr Sayera Banu email@example.com
Evalution of Xpert Ultra for the diagnosis of childhood pulmonary TB using stool specimen
Evalution of Xpert Ultra for the diagnosis of childhood pulmonary TB using stool specimen findings presentation
Scientific paper on Xpert Ultra assay on stool to diagnose pulmonary tuberculosis in children
A brief on the evaluation of Xpert MTB/RIF Ultra for the diagnosis of childhood pulmonary tuberculosis using stool specimen study